This project was funded by the KSPB grant program in 2019-2020, which allowed us to obtain exciting data related to the use of the biocontrol agent Trichoderma in regulating soybean growth and development as well as response to biotic and abiotic stresses. Our work has identified plant produced chemicals that can provide pathogen resistance and abiotic tolerance in soybean. In addition, we showed that Trichoderma positively regulates Rhizobia-mediated nitrogen fixation. A patent application involving the use of a recently discovered RNA molecule to improve biotic resistance has been filed. Funding is requested for an additional year to complete the field trials using Trichoderma and to develop the patented RNA molecule for field trial testing. The overall goal of this research project is to increase profitability of soybean production by simultaneously increasing yield as well as tolerance against biotic and abiotic stresses.