Meloidogyne enterolobii is an introduced root-knot nematode species to North Carolina. This species has a broad host range (including many vegetables, field crops, and weeds) and threatens profitable soybean production in this state by causing severe root galling. M. enterolobii is more aggressive than the other endemic root-knot nematode species found in NC. Management of this species through cultural and chemical control is difficult, as it appears to increase population densities to damaging levels even with fumigation. Chemical controls for general nematode control in soybean are limited due to the costs of application and the limited economic return with their application. Identification of varietal resistance is important to continue to economically produce soybean with this pest continuing to spread in North Carolina. Previous screening of the Official Variety Test soybean varieties resulted in no resistance to M. enterolobii being found. This project will screen selected PI lines (n=80) with known resistance to other root-knot nematode species and the soybean cyst nematode to assess potential mechanisms for resistance as well as identify the sensitivity of soybean lines to this new root knot nematode. In addition to identifying resistant varieties, nematodes are frequently managed through cover cropping with non-hosts. Several small grain cover crops have been reported as resistant or non-host to M. enterolobii, however the utility of using cover crops to suppress M. enterolobii is not well understood. This project will screen several NC cover crops for their host status to M. enterolobii as well as their effectiveness in suppressing M. enterolobii in soil, thus providing a benefit to a following soybean crop.